Health Sciences Sequencing Core
All PITT/UPMC prices below are effective March 1, 2023.
| Sample Preparation | Unit | Price |
| RNA/DNA extraction tissue | Sample | $32 |
| RNA/DNA extraction biofluid (PaxGene, blood) or FFPE | Sample | $47 |
| Quality checks | Unit | Price |
| Agilent TapeStation DNA or RNA | Sample | $9 |
| Qubit Fluorometric Quantification | Sample | $5 |
| RNA Library preparation | Unit | Price |
| llumina Stranded Total Ligation (Ribo-Zero Plus) | Sample | $240 |
| llumina Stranded Total Ligation (Ribo-Zero Plus) ≥ 48 Samples | Sample | $200 |
| llumina Stranded mRNA (PolyA+) 1-47 | Sample | $135 |
| llumina Stranded mRNA (PolyA+) ≥ 48 Samples | Sample | $110 |
| SMARTer TCR Profiling (Human / mouse) | Sample | $145 |
| SMART-Seq Stranded Total RNA (Ultra Low Input) | Sample | $150 |
| SMART-Seq Stranded Total RNA (Ultra Low Input) ≥ 48 Samples | Sample | $125 |
| SMART-Seq HT Ultra Low Input | Sample | $105 |
| QIAseq small RNA | Sample | $220 |
| Nanostring GeoMX (per plate) | Sample | $190 |
| High-capacity library pooling | Sample | $15 |
| Visium Spatial Transcriptomics | Unit | Price |
| Visium Tissue RNA QC | Sample | $25 |
| Visium Fresh Frozen Tissue Permeabilization | Sample | $1,200 |
| Visium Fresh Frozen Spatial Gene Expression | Sample | $6,200 |
| Visium FFPE v2 Spatial Gene Expression, 6.5mm capture | Sample | $8,400 |
| Visium FFPE v2 Spatial Gene Expression, 11mm capture | Sample | $8,600 |
| DNA Library Preparation | Unit | Price |
| Nextera DNA XT (low input) | Sample | $105 |
| Illumina DNA Prep (Nextera FLEX) | Sample | $110 |
| Illumina DNA PCR-Free Prep (WGS) | Sample | $110 |
| ChIP-seq (Library prep only) | Sample | $158 |
| ATAC-Seq | Sample | $262 |
| NextSeq 2000 Sequencing Runs | Unit | Price |
| P1 300 cycle kit (100M) | Run | $1,800 |
| P2 100 cycle kit (400M) | Run | $1,900 |
| P2 200 cycle kit (400M) | Run | $3,450 |
| P2 300 cycle kit (400M) | Run | $4,480 |
| P3 50 cycle kit (1200M) | Run | $3,100 |
| P3 100 cycle kit (1200M) | Run | $4,000 |
| P3 200 cycle kit (1200M) | Run | $5,600 |
| P3 300 cycle kit (1200M) | Run | $7,350 |
| MiSeq Sequencing Runs | Unit | Price |
| MiSeq 50 cycle kit (15M) | Run | $1,180 |
| MiSeq 150 cycle kit (25M) | Run | $1,280 |
| MiSeq 300 cycle kit (15M) | Run | $1,460 |
| MiSeq v3 600 cycle kit (25M) | Run | $2,050 |
| MiSeq v2 300 cycle kit MICRO (4M) | Run | $620 |
| MiSeq v2 300 cycle kit NANO (1M) | Run | $510 |
| Special Services | Unit | Price |
| Special Analysis/Services | Hour | $60 |
For external customers, please contact Health Sciences Sequencing Core directly
Single Cell Core
All prices below are effective January 1, 2024.
|
10X Genomics Single Cell Library Preparation |
Unit |
Price |
|
3’ or 5’ Single Cell RNA-Seq (Base-1st well) |
Sample |
$3,251 |
|
3’ or 5’ Single Cell RNA-Seq (2nd-8th well) |
Sample |
$2,090 |
|
Single Cell ATAC-Seq (Base-1st well) |
Sample |
$3,189 |
|
Single Cell ATAC-Seq (2nd-8th well) |
Sample |
$2,165 |
|
Single Cell Multiome (Base-1st well) |
Sample |
$3,626 |
|
Single Cell Multiome (2nd-8th well) |
Sample |
$3,319 |
|
Single Cell FLEX (Fixed RNA Single-Plex) per sample |
Reaction |
$2,352 |
|
Single Cell FLEX (Fixed RNA 4-Plex) per 4 samples |
Reaction |
$4,182 |
|
Single Cell FLEX (Fixed RNA 16-Plex) per 16 samples |
Reaction |
$10,809 |
|
Additional Library Preparation |
Unit |
Price |
|
BCR/TCR Library, mouse or human (add on to 5’ scRNAseq) |
Sample |
$321 |
|
Feature Barcoding (Cite-Seq or hashtagging for 3’ and 5’ scRNAseq) |
Sample |
$326 |
|
Library QC |
Unit |
Price |
|
Qubit Quantification |
Sample |
$7 |
|
TapeStation cDNA and Library QC |
Sample |
$32 |
Please contact heidi.monroe@pitt.edu for a project quote.